Eukaryotic protein biosynthesis research group

We are interested in understanding the protein synthesis machinery in eukaryotes. The genetic information encoded in messenger RNA is translated into proteins by large ribonucleoprotein complex called ribosomes. The main research question of our studies is: How the function of eukaryotic ribosomes is determined by their structure?

To answer this question, we use genetic, biochemical and mass spectrometric approaches in budding yeast (Saccharomyces cerevisiae). In ongoing research, we explore the role of three eukaryote-specific ribosomal proteins that participate in formation of contacts between ribosomal subunits. These contacts, called intersubunit bridges, ensure correct interaction and communication of ribosomal subunits during translation initiation and elongation steps. Using yeast mutants, we investigate how the lack of eukaryote-specific bridges affects the processivity of ribosomes, fidelity of decoding and recognition of different mRNA elements such as upstream open reading frames (uORF), internal ribosome entry sites (IRES) and programmed ribosomal frameshift signals. We study how cells carrying these mutant ribosomes tolerate different stress conditions and how the pattern of the protein synthesis changes in these cells.

Kisly, I., Kattel, C., Remme, J. Tamm, T. (2021) Luciferase-based reporter system for in vitro evaluation of elongation rate and processivity of ribosomes. Nucleic Acids Res. 49(10):e59. doi: 10.1093/nar/gkab121

Tamm T, Kisly I, Remme J. (2019) Functional Interactions of Ribosomal Intersubunit Bridges in Saccharomyces cerevisiae. Genetics 213(4):1329-1339. doi: 10.1534/genetics.119.302777

Kisly I, Remme J, Tamm T. (2019) Ribosomal protein eL24, involved in two intersubunit bridges, stimulates translation initiation and elongation. Nucleic Acids Res. 47(1):406-420. doi: 10.1093/nar/gky1083

(in Estonian)

Novaator: Tartu Ülikooli teadlased tegid elu alusprotsesse uurides tähtsa avastuse

Kuku raadio:Teadus teab

Kuku raadio: Kuue samba taga. Mida uurivad molekulaarbioloogid ning kuidas nende töö aitab ravimitootjaid ja veinitegijaid? Saatekülaline on molekulaar- ja rakubioloogia instituudi professor Tiina Tamm.

Novaator: Gümnasistid tegid koos teadlastega kindlaks Eestis kasvavad pärmseened

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Research and Development Projects


IMCB in-house seminars: spring 2023

MR Angela Ivask teadusgrupp Autor Aleksandr Käkinen

Microbe & material interactions research group