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ResearchChair of Developmental Biology: Apicobasal polarity determinants are potential tumor suppressors that have been extensively studied. However, the precise mechanisms by which their misregulation disrupts tissue homeostasis are not fully understood. A comprehensive protocol is presented for establishing a conditional RNAi knockdown of scribble in Drosophila wing imaginal disc. Steps for generating fly lines, conditional knockdown of flies, and sample preparation are described. Detail procedures for imaging, image analysis, and verification of wing disc phenotypes by various antibodies are given.
Conditional knockdown protocol for studying cellular communication using Drosophila melanogaster wing imaginal discChair of Cell Biology: Initiation of DNA replication in eukaryotes is timed according to cell cycle phase. which is influenced by several factors. In budding yeast, Forkhead family proteins Fkh1 and Fkh2 bind to replication origins and activate them in early S phase. The binding sites of these proteins are in a specific configuration, suggesting that the hammerhead proteins must also bind in a specific manner. The article shows which regions of the intermolecular interaction are important for binding and which molecular mechanisms ensure the necessary regulation.
Interactions between Fkh1 monomers stabilize its binding to DNA replication originsChair of Genetics: The effect of microorganisms on the decomposition of organic matter in black shale (graptolite argillite), as well as on the leaching and/or precipitation of metals (V, Mo, U and Zn) was studied. Graptolite argillite in the bedrock of Estonia is a habitat for microbes (inactive cells or spores). For the first time, the research combined two seemingly contradictory goals: firstly, to use the rock as a source of methane generation and secondly, as a metal ore. This was achieved thanks to the thorough competence in environmental microbiology and genetics of IMCB's scientists.
Methanogenesis and metal leaching on anaerobic decomposition of graptolite argilliteToday