Most bacteria live in biofilm, at least for some stage of life. We study the attachment of the cosmopolitan bacterium Pseudomonas putida, to the surface and the regulation of biofilm.
FleQ, a two-component system of GacS/A, a global second messager of cdGMP, Fis, etc., is known to be involved in regulating P. putida biofilm. Fis is one of the global transcriptional regulators that affects bacterial physiology, including biofilm and cell surface properties. We have found that Fis reduces cell surface hydrophobicity by repressing lapF transcription. Fis enhances biofilm by activating lapA transcription and activating NADH dehydrogenase genes' expression, the first component of the respiratory chain.
We currently study the cross-regulation of biofilm regulators and how Fis affects GacS/A pathway components and the regulation of gacS expression by excludon, which is the function of diversely transcribed mRNAs as asRNA. In addition, environmental factors influencing the formation of P. putida biofilm are considered.
Scheme used in the header: Regulation of biofilm formation in Pseudomonas putida (author: Riho Teras)
Puhm, M., Hendrikson, J., Kivisaar, M., & Teras, R. (2022). Pseudomonas putida Biofilm Depends on the vWFa-Domain of LapA in Peptides-Containing Growth Medium. International journal of molecular sciences, 23(11), 5898.
Puhm, M., Ainelo, H., Kivisaar, M., & Teras, R. (2022). Tryptone in Growth Media Enhances Pseudomonas putida Biofilm. Microorganisms, 10(3), 618.
Teppo A, Lahesaare A, Ainelo H, Samuel K, Kivisaar M, et al. (2018) Colonization efficiency of Pseudomonas putida is influenced by Fis-controlled transcription of nuoA-N operon. PLoS One 13: e0201841
Ainelo, H., A. Lahesaare, A. Teppo, M. Kivisaar & R. Teras, (2017) The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. PloS one 12: e0185482
Lahesaare, A., H. Ainelo, A. Teppo, M. Kivisaar, H.J. Heipieper & R. Teras, (2016) LapF and Its Regulation by Fis Affect the Cell Surface Hydrophobicity of Pseudomonas putida. PloS one 11: e0166078
Teras, Riho (2019). Bakterite planeet - biokile planeet. Eesti Loodus, 10, 54−58.
Mikrobioloogia tutvustamine põhikoolilastele (2020 Võhma põhikool, 2021 TÜMRIs)