Meelis Kadaja külalisseminar 9.09.13

Meelis Kadaja (Laboratory Of Mammalian Cell Biology And Development, The Rockefeller University, New York, USA) peab 09.09.2013 kl 10.00 Riia 23b-105 külalisseminari teemal:
"Deciphering the mechanisms governing adult stem cell maintenance in skin"

Short introduction:

Stem cells (SCs) have the ability to self-renew long term and differentiate along one or more lineages. The hair follicle serves as a useful model in SC biology since it undergoes periodic cycles of growth, degeneration and rest, which is fueled by SCs at the base of the non-cycling part of the follicle (bulge). Sox9 is an essential bulge SC transcription factor, but how it governs SC function is unknown. In order to determine how Sox9 regulates the maintenance of adult SCs during tissue homeostasis, we have isolated functional bulge SCs and identified by chromatin immunoprecipitation and deep sequencing, which genes bind Sox9 directly and whether they are in a chromatin active or repressed state. We have also performed RNA-sequencing to elucidate how SC expression program changes when Sox9 is absent. By combining these two datasets, we identified and validated the most interesting Sox9 target genes and investigated their roles in SC maintenance.